Journal: British Journal of Pharmacology

Article Title: Distinct effects of CGRP on typical and atypical smooth muscle cells involved in generating spontaneous contractions in the mouse renal pelvis

doi: 10.1111/j.1476-5381.2009.00514.x

Figure Lengend Snippet: Distribution of PGP9.5-, calcitonin gene-related peptide (CGRP)- and transient receptor potential cation channel, subfamily V, member 1 (TRPV1) immunoreactive nerves in the mouse renal pelvis. In the proximal renal pelvis, nerve fibres were stained with antibodies against the neural marker PGP 9.5 (Aa). CGRP-immunoreactive nerve fibres were abundant in the same preparation (Ab). A merged image indicates that most of thick and thin nerve bundles contain CGRP-immunoreactive fibres (Ac). Double staining of the mid renal pelvis with TRPV1 (Ba) and CGRP (Bb) antibodies showed that many CGRP-positive fibres also expressed TRPV1-immnoreactivity (Bc). Scale bars: 80 µm.

Article Snippet: To examine colocalization of CGRP and transient receptor potential cation channels (TRPV1; vanilloid receptors), whole mounts were incubated with rabbit anti-CGRP antibody (1:50, 11189, Progen Biotechnik, Heidelberg, Germany) and guinea pig anti-TRPV1 antibody (1:1000, GP14100, Neuromics, Edina, MN, USA) for 2 days at 4°C.

Techniques: Staining, Marker, Double Staining

Journal: Molecular Neurobiology

Article Title: Spared Nerve Injury Causes Sexually Dimorphic Mechanical Allodynia and Differential Gene Expression in Spinal Cords and Dorsal Root Ganglia in Rats

doi: 10.1007/s12035-021-02447-1

Figure Lengend Snippet: L4 dorsal root ganglion (DRG) immunostaining for IB-4 (a) and CGRP (b) after SNI in male (M) and female (F) rats and representative images of staining in L4 (c). **** p < 0.001, ** p < 0.01 by Student’s t -test with Holm-Sidak correction for multiple comparisons and ANOVA. ## p < 0.01 by ANOVA. n = 10

Article Snippet: DRG sections were probed with antibodies for CGRP (1:10,000, Cat# T-4032, Peninsula Laboratories, San Carlos, CA, USA) or FITC-conjugated IB-4 (1:200; Cat# FL-1201, Vector Labs, CA, USA).

Techniques: Immunostaining, Staining